Biodecontamination of biosafety cabinets

Biodecontamination of biosafety cabinets


We provide VH2O2decontamination services for the phama industry and life sciences such as the application in biosafety cabinets.

For many years, the use of formaldehyde gas has been the most common methodology but due to the adverse effects over health of operators (carcinogen risks), the use of formalin has been banned and the best alternative is the use of VH2O2.

The method requires the use of the DosyMist®VH2O2generator with a remote spray head and some accessories specifically developed for this application.

The target is to decontaminate all the spaces and internal surfaces of the cabinet, trying to achieve a level of decontamination of 6-log.

In order to check the efficiency of the cycle, our services include chemical indicators, biological indicators and/or Protak ™ Enzymatic Indicators.

When is it required to perform a complete decontamination of a Biological Safety Cabinet?

  • Before any repair, especially if it involves accessing the contaminated plenum, change of filters, blowers, etc.
  • When the cabinet needs to be replaced or relocated from one room or building to another
  • When the working procedure (e.g. type of microorganism) has to be drastically changed
  • Right after a serious spillage of a dangerous micro-organism has happened
  • If there’s suspicion of product contamination by the cabinet itself
  • Periodically, especially when BSL-3 or BSL-4 classified organisms are being manipulated inside the cabinet

In such cases, the decontamination should be performed in vapour phase in order to reach out and contact all inner surfaces and parts of the cabinet, including the motor-fan and HEPA filtration systems, which are not typically accessible for disinfection with liquid chemicals.

Advantages of VH2O2 versus Formalin:

  • VH2O2is not posing any risk for operators and workers in the area (no carcinogenic)
  • Permissible levels of 1 ppm is relatively affordable and risks are still fair until 75 ppm
  • It is not necessary to have a complete airtight confinement (no need to use bags). Some small gaps are permissible
  • People can still work in the lab. Having a H2O2 detector around the area is recommended.
  • It leaves no residues
  • Cycle times depend on availability of connecting to the exhaust and/or use of catalytic elements but it is typically much shorter than formaldehyde (3-12 hours)